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KMID : 1149220090170020151
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Korean Journal of Oriental Medical Prescription
2009 Volume.17 No. 2 p.151 ~ p.162
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Anti-inflammatory effects of Herba Artemisiae Capillaris as a consequence of the inhibition of NF-kappa B-dependent iNOS and pro-inflammatory cytokines production
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Kim Dae-Sung
Park Sook-Jahr
Jo Mi-Jeong
Park Sang-Mi
Kim Sang-Chan
Byun Sung-Hee
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Abstract
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Herba Artemisiae Capillaris is the dried bud of Artemisia capillaris Thunb, which has been used for expelling heat to loosen the bowels and normalizing gallbladder function to cure jaundice in traditional oriental medicines. In the present study, we evaluated the anti-inflammatory effects of the aqueous extracts of Herba Artemisiae Capillaris (HAC) in LPS-activated Raw 264.7 cells. Cells were treated with 1 μg/ml of LPS 1 h before adding HAC extract. Cell viability was determined by MTT assay, and the relative level of NO was measured with Griess reagent. TNF-α, IL-1β, and IL-6 cytokines were detected by ELISA. During the entire experimental period, all three doses of HAC extract (0.03, 0.10 and 0.30 mg/ml) had no significant cytotoxicity. LPS-activated cells showed increased NO levels and iNOS expressions compared to control. However, these increases were dramatically attenuated by treatment with HAC extract. Moreover, the inhibitory effects of HAC extract occurred in a dose-dependent manner. In addition, HAC extract reduced the translocation of NFκB into nuclear. HAC reduced production of IL-1β and IL-6 by LPS, although it had no effects on TNF-α. These results demonstrate that liquiritigenin exerts anti-inflammatory effects, which results from the inhibition of NFκB activation in macrophages, thereby decreasing production of iNOS and proinflammatory cytokines. Taken together, these results indicate that the aqueous extracts of Herba Artemisiae Capillaris warrant further development as an anti-inflammatory agent for the treatment of gram-negative bacterial infections.
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KEYWORD
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Herba Artemisiae Capillaris, Cytokine, iNOS, NFκB
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